Establishing and expanding molecular HIV diagnostics


Swiss TPH
Department Biomedizin Basel Butha-Buthe District Hospital
Ministry of Health

Health system impact

Health Workforce: Over 120 health care providers in the two districts were trained and re-trained on the clinical implications of regular viral load monitoring and in the management of treatment failure; 6 Laboratory technologists from Butha-Buthe and Seboche received a 5-day onsite advanced viral load testing training at the state of the art Laboratory of Roche Diagnostics in Johannesburg in South Africa

Service Delivery: 25,686 viral load tests for 15,548 patients living with HIV using new state-of-the-art molecular diagnostics enabling clinicians to monitor treatment effectiveness; 271 patients were switched to a second-line regimen of antiretroviral therapy according to the national guidelines; more than 90% of persons living with HIV in the district received at least one viral load test. The Seboche laboratory has been renovated and became the first laboratory in Lesotho to provide HIV drug resistance testing.

Scientific publications

Publication: The viral load monitoring cascade in a resource-limited setting: A prospective multicentre cohort study after introduction of routine viral load monitoring in rural Lesotho, by Tracy Renee Glass, Lipontso Motaboli, Bienvenu Nsakala, Malebanye Lerotholi, Fiona Vanobberghen, Alain Amstutz, Thabo Ishmael Lejone, Josephine Muhairwe, Thomas Klimkait, Niklaus Daniel Labhardt, PLoS One, August 28, 2019

A second manuscript focusing on virologic outcomes of children/adolescents is currently in preparation and will be submitted to the Pediatric Infectious Disease Journal.

Read more about this partnership here.